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Anti-CD44 Antibody (5V292) is an antibody targeting CD44. Anti-CD44 Antibody (5V292) can be used in ELISA, FC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Anti-CD44 Antibody (5V292) is an antibody targeting CD44. Anti-CD44 Antibody (5V292) can be used in ELISA, FC. |
Alias | Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group) |
Ig Type | Mouse IgG2a |
Clone | 5V292 |
Reactivity | Human |
Verified Activity | Overlay Peak curve showing Hela cells surface stained with TMAH-00201 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Application | ELISA, FC |
Recommended Dose | FC:1:20-1:500. |
Antibody Type | Monoclonal |
Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human CD44 Protein |
Antigen Species | Human |
Gene ID | 960 |
Uniprot ID | |
Biology Area | Cancer, Immunology, Stem cells |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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