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Anti-Neuraminidase Protein, C.perfringens Antibody (8O474) is a Mouse antibody targeting Neuraminidase Protein, C.perfringens. Anti-Neuraminidase Protein, C.perfringens Antibody (8O474) can be used in WB,ELISA,IHC-P,FCM,ICC/IF,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | 88 € | 7-10 days | |
100 μL | 254 € | 7-10 days |
Description | Anti-Neuraminidase Protein, C.perfringens Antibody (8O474) is a Mouse antibody targeting Neuraminidase Protein, C.perfringens. Anti-Neuraminidase Protein, C.perfringens Antibody (8O474) can be used in WB,ELISA,IHC-P,FCM,ICC/IF,IP. |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 8O474 |
Reactivity | C.perfringens |
Application | WB,ELISA,IHC-P,FCM,ICC/IF,IP |
Recommended Dose | WB: his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect Clostridium Perfringens Neuraminidase in WB. Using a DAB detection system, the detection limit for Clostridium Perfringens Neuraminidase is approximately 1 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.; ELISA: 1:1000-1:2000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Clostridium Perfringens Neuraminidase (TMPY-01468; P10481.1; Cys 2-Gln 382). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Clostridium perfringens / C. perfringens (formerly known as C. welchii) is a Gram-positive, rod-shaped, anaerobic, spore-forming bacterium of the genus Clostridium. C. perfringens is ubiquitous in nature and can be found as a normal component of decaying vegetation, marine sediment, the intestinal tract of humans and other vertebrates, insects, and soil. C. perfringens is commonly encountered in infections as a benign component of the normal flora. In this case, its role in disease is minor. Infections due to C. perfringens show evidence of tissue necrosis, bacteremia, emphysematous cholecystitis, and gas gangrene, which is also known as clostridial myonecrosis. NA, also called sialidases, specifically catalyze the hydrolysis removal of terminal sialic acid residues from viral and cellular glycoconjugates. C. Perfringens neuraminidase catalyzes the hydrolysis of alpha-(2->3)-, alpha-(2->6)-, glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates, but has little activity against the α2-8 glycosidic linkages. The function of the neuraminidase is to release sialic acids for use as carbon and energy sources for the non-pathogenic bacterium, while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors |
Conjucates | Unconjugated |
Immunogen | Recombinant Clostridium Perfringens Neuraminidase Protein (TMPY-01468) |
Antigen Species | C.perfringens |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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