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Synonyms: ZTNF-2, ZTNF2, ZTNF 2, TRDL-1, TRDL1, TRDL 1, TNFSF-13, TNFSF13, TNFSF 13, TALL-2, TALL2, TALL 2, CD-256, CD256, CD 256, APRILFLJ-57090, APRILFLJ57090, APRILFLJ 57090, APRIL, 2310026N09Rik


| Bioactivity | Immobilized Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag at 1 μg/ml (100 μl/Well) on streptavidin (5 μg/ml) precoated plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 2.0 ng/ml determined by ELISA. |
| Description | APRIL/TNFSF13 Trimer Protein, Cynomolgus, Recombinant (His & Avi, Flag), Biotinylated is expressed in HEK293 Cells. The accession number is A0A2K5TJA1. |
| Species | Cynomolgus |
| Expression System | HEK293 Cells |
| Tag | N-His-Avi-Flag |
| Accession Number | A0A2K5TJA1 |
| Construction | Lys112-Leu250 trimer design |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | ZTNF-2, ZTNF2, ZTNF 2, TRDL-1, TRDL1, TRDL 1, TNFSF-13, TNFSF13, TNFSF 13, TALL-2, TALL2, TALL 2, CD-256, CD256, CD 256, APRILFLJ-57090, APRILFLJ57090, APRILFLJ 57090, APRIL, 2310026N09Rik |
| Research Background |
| Molecular Weight | 52.1 kDa (Predicted); 56-65 kDa (Reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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