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Anti-SKP2 Antibody (5S383)
Catalog No. TMAH-01095 Copy Product Info
🥰Excellent
Anti-SKP2 Antibody (5S383) is an antibody targeting SKP2. Anti-SKP2 Antibody (5S383) can be used in ELISA,IF,IHC.
Anti-SKP2 Antibody (5S383)
Copy Product Info🥰Excellent
Catalog No. TMAH-01095
Anti-SKP2 Antibody (5S383) is an antibody targeting SKP2. Anti-SKP2 Antibody (5S383) can be used in ELISA,IF,IHC.
Anti-SKP2 Antibody
(5S383)
(5S383)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $207 | 7-10 days | 7-10 days | |
| 100 μL | $347 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
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| Description | Anti-SKP2 Antibody (5S383) is an antibody targeting SKP2. Anti-SKP2 Antibody (5S383) can be used in ELISA,IF,IHC. |
| Ig Type | Rabbit IgG |
| Clone | 5S383 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-01095 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. Immunofluorescence staining of Hela Cells with TMAH-01095 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | |
| Recommended Dose | IHC:1:50-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration. Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human SKP2 |
| Antigen Species | Human |
| Gene Name | SKP2 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cancer, Cardiovascular, Cell biology |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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