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Anti-Phospho-RPS6KA5 (Ser360) Polyclonal Antibody
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Catalog No. TMAC-02655
Anti-Phospho-RPS6KA5 (Ser360) Polyclonal Antibody is a Rabbit antibody targeting Phospho-RPS6KA5 (Ser360). Anti-Phospho-RPS6KA5 (Ser360) Polyclonal Antibody can be used in IHC-P,ICC/IF,ELISA.
Anti-Phospho-RPS6KA5 (Ser360) Polyclonal Antibody
Anti-Phospho-RPS6KA5 (Ser360) Polyclonal Antibody
😃Good
Catalog No. TMAC-02655
Anti-Phospho-RPS6KA5 (Ser360) Polyclonal Antibody is a Rabbit antibody targeting Phospho-RPS6KA5 (Ser360). Anti-Phospho-RPS6KA5 (Ser360) Polyclonal Antibody can be used in IHC-P,ICC/IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $216 | 7-10 days | |
| 100 μL | $316 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-RPS6KA5 (Ser360) Polyclonal Antibody is a Rabbit antibody targeting Phospho-RPS6KA5 (Ser360). Anti-Phospho-RPS6KA5 (Ser360) Polyclonal Antibody can be used in IHC-P,ICC/IF,ELISA. |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Application | |
| Recommended Dose | IHC-P: 1:100-300; ICC/IF: 1:200-1000; ELISA: 1:10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Appearance | Liquid |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research Background | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.,function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).,miscellaneous:Enzyme activity requires the presence of both kinase domains.,PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subcellular location:Predominantly nuclear. Partially cytoplasmic.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.,tissue specificity:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver., |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human MSK1 around the phosphorylation site of Ser360. AA range:331-380 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 89 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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