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Anti-Ferritin light chain Antibody (7V55)

😃Good
Catalog No. TMAH-00462
Alias NBIA3, MGC71996, LFTD, ferritin, light polypeptide

Anti-Ferritin light chain Antibody (7V55) is an antibody targeting Ferritin light chain. Anti-Ferritin light chain Antibody (7V55) can be used in ELISA, IHC, FCM.

Anti-Ferritin light chain Antibody (7V55)

Anti-Ferritin light chain Antibody (7V55)

😃Good
Catalog No. TMAH-00462Alias NBIA3, MGC71996, LFTD, ferritin, light polypeptide
Anti-Ferritin light chain Antibody (7V55) is an antibody targeting Ferritin light chain. Anti-Ferritin light chain Antibody (7V55) can be used in ELISA, IHC, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IHC, FCM

Reactivity: Human
AliasNBIA3, MGC71996, LFTD, ferritin, light polypeptide
Ig Type
Rabbit IgG
Clone
7V55
Reactivity
Human
Verified Activity
1. IHC image of TMAH-00462 diluted at 1:50 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.29% DAB.
2. Overlay Peak curve showing MCF-7 cells stained with TMAH-00462 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, IHC, FCM
Recommended Dose
IHC:1:50-1:200; FCM:1:50-1:200.
Antibody Type
Monoclonal
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human FTL
Antigen Species
Human
Gene ID
2512
Uniprot ID
Biology Area
Neuroscience, Cancer, Cardiovascular, Metabolism, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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