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Anti-NPY1R Polyclonal Antibody

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Catalog No. TMAB-01259

Anti-NPY1R Polyclonal Antibody is a Rabbit antibody targeting NPY1R. Anti-NPY1R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-NPY1R Polyclonal Antibody

Anti-NPY1R Polyclonal Antibody

😃Good
Catalog No. TMAB-01259
Anti-NPY1R Polyclonal Antibody is a Rabbit antibody targeting NPY1R. Anti-NPY1R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NPY1R Polyclonal Antibody is a Rabbit antibody targeting NPY1R. Anti-NPY1R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Rabbit)
Verified Activity
1. Tissue/cell: rat myocardium tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NPY1R Polyclonal Antibody, Unconjugated (TMAB-01259) 1:400, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. Sample:
Lane 1: Mouse NIH/3T3 tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Human 293T cell lysates
Primary: Anti-NPY1R (TMAB-01259) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa
Application
WB,IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNeuropeptide Y (NPY) is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system. There are two major subtypes of NPY (Y1 and Y2). The NPY Y1 receptors have been identified in a variety of tissues, including brain, spleen, small intestine, kidney, testis, placenta, breast, colon, ganglion, heart, and aortic smooth muscle. The Y2 receptor is found mainly in the central nervous system. NPY receptors are a member of the G protein-coupled receptor superfamily.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NPY1R
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Blood Pressure regulation,Vasodilation,GPCR
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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