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Anti-NPY1R Polyclonal Antibody is a Rabbit antibody targeting NPY1R. Anti-NPY1R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days |
Description | Anti-NPY1R Polyclonal Antibody is a Rabbit antibody targeting NPY1R. Anti-NPY1R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Rabbit) |
Verified Activity | 1. Tissue/cell: rat myocardium tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-NPY1R Polyclonal Antibody, Unconjugated (TMAB-01259) 1:400, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. 2. Sample: Lane 1: Mouse NIH/3T3 tissue lysates Lane 2: Rat Cerebrum tissue lysates Lane 3: Rat Kidney tissue lysates Lane 4: Human 293T cell lysates Primary: Anti-NPY1R (TMAB-01259) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 60 kDa |
Application | WB,IHC-P,IHC-Fr,IF,ELISA |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Neuropeptide Y (NPY) is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system. There are two major subtypes of NPY (Y1 and Y2). The NPY Y1 receptors have been identified in a variety of tissues, including brain, spleen, small intestine, kidney, testis, placenta, breast, colon, ganglion, heart, and aortic smooth muscle. The Y2 receptor is found mainly in the central nervous system. NPY receptors are a member of the G protein-coupled receptor superfamily. |
Immunogen | KLH conjugated synthetic peptide: human NPY1R |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Blood Pressure regulation,Vasodilation,GPCR |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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