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Synonyms: VEGI192A, VEGI, TNLG1B, TL1A, TL1, MGC129935, MGC129934


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $242 | 7-10 days | 7-10 days | |
| 10 μg | $388 | 7-10 days | 7-10 days | |
| 20 μg | $621 | - | In Stock | |
| 50 μg | $994 | 7-10 days | 7-10 days | |
| 100 μg | $1,433 | 7-10 days | 7-10 days |
| Bioactivity | 1. Immobilized DcR3 Protein, Human, Recombinant (hFc) (Cat#TMPY-03521) at 2 μg/mL (100 μL/well) can bind TL1A/TNFSF15 Trimer Protein, Human, Recombinant (His & Avi), Biotinylated (Cat#TMPY-07145), the EC50 is 1-3 ng/mL. 2. Loaded TL1A/TNFSF15 Trimer Protein, Human, Recombinant (His & Avi), Biotinylated (Cat#TMPY-07145) on SA Biosensor, can bind DR3 Protein, Human, Recombinant (hFc) (Cat#TMPY-01751) with an affinity constant of 2.57 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Immobilized Anti-TNFSF15 Antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind TL1A/TNFSF15 Trimer Protein, Human, Recombinant (His & Avi), Biotinylated (Cat#TMPY-07145), the EC50 is 22.1 ng/mL(Routinely tested). |
| Description | TL1A/TNFSF15 Trimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is O95150-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | N-His-Avi |
| Accession Number | O95150-1 |
| Construction | A DNA sequence encoding the Human TL1A / TNFSF15 (NP_005109.2) (Leu72-Leu251) was expressed with a N-terminal polyhistidine tag followed by an AVI tag.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. This construct has a trimer design. |
| Protein Purity | ≥ 95% as determined by SDS-PAGE. ≥ 90% as determined by SEC-MALS. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Please refer to the lot-specific COA. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | VEGI192A, VEGI, TNLG1B, TL1A, TL1, MGC129935, MGC129934 |
| Research Background |
| Molecular Weight | 68.51 kDa (predicted); 69.4 kDa (reducing contition, due to glycosylation); 87.3 kDa (SEC-MALS) |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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