SARS-CoV-2 Spike S1+S2 trimer Protein (His & Avi), Biotinylated

Name: SARS-CoV-2 Spike S1+S2 trimer Protein (His & Avi), Biotinylated
Brand: TargetMol
SKU: TMPY-06455
Rating: 4.5 (1 reviews)

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Catalog No. TMPY-06455 Copy Product Info

SARS-CoV-2 Spike S1+S2 trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 138.28 kDa and the accession number is YP_009724390.1.

Pack Size	Price	USA Stock	Global Stock
5 μg	$250	7-10 days	7-10 days
10 μg	$419	7-10 days	7-10 days
20 μg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized human ACE2 protein (mFc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1+S2 trimer Protein (ECD, His & AVI Tag), Biotinylated, the EC50 is 10-48 ng/mL.
Description	SARS-CoV-2 Spike S1+S2 trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 138.28 kDa and the accession number is YP_009724390.1.
Species	SARS-CoV-2
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	YP_009724390.1
Construction	A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations D614G,F817P, A892P, A899P,A942P, K986P, V987P and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Val 16
Protein Purity	> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a sodium citrate buffer system at pH 6. 0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background

Chemical Properties

Molecular Weight

138.28 kDa (predicted)

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: SARS-CoV-2 Spike S1+S2 trimer Protein (His & Avi), Biotinylated chemical structure | SARS-CoV-2 Spike S1+S2 trimer Protein (His & Avi), Biotinylated molecular weight