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Anti-NDUFS3 Antibody (2K991) is an antibody targeting NDUFS3. Anti-NDUFS3 Antibody (2K991) can be used in ELISA, WB, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Anti-NDUFS3 Antibody (2K991) is an antibody targeting NDUFS3. Anti-NDUFS3 Antibody (2K991) can be used in ELISA, WB, IHC. |
Alias | NDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD |
Ig Type | Rabbit IgG |
Clone | 2K991 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, -All lanes: NDUFS3 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 31, 15 KDa -Observed band size: 31 kDa 2. IHC image of TMAH-00807 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
Application | ELISA, WB, IHC |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human NDUFS3 |
Antigen Species | Human |
Gene ID | 4722 |
Uniprot ID | |
Biology Area | Cancer, Cell biology, Metabolism, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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