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Anti-NDUFS3 Antibody (2K991)

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Catalog No. TMAH-00807
Alias NDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD

Anti-NDUFS3 Antibody (2K991) is an antibody targeting NDUFS3. Anti-NDUFS3 Antibody (2K991) can be used in ELISA, WB, IHC.

Anti-NDUFS3 Antibody (2K991)

Anti-NDUFS3 Antibody (2K991)

😃Good
Catalog No. TMAH-00807Alias NDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD
Anti-NDUFS3 Antibody (2K991) is an antibody targeting NDUFS3. Anti-NDUFS3 Antibody (2K991) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NDUFS3 Antibody (2K991) is an antibody targeting NDUFS3. Anti-NDUFS3 Antibody (2K991) can be used in ELISA, WB, IHC.
AliasNDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD
Ig Type
Rabbit IgG
Clone
2K991
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate,
-All lanes: NDUFS3 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 31, 15 KDa
-Observed band size: 31 kDa
2. IHC image of TMAH-00807 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationMitochondrion inner membrane; Peripheral membrane protein; Matrix side.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human NDUFS3
Antigen Species
Human
Gene ID
4722
Uniprot ID
Biology Area
Cancer, Cell biology, Metabolism, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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