B7-H4 Protein, Human, Recombinant (His)

Name: B7-H4 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-03524
Rating: 4.5 (1 reviews)

(Synonyms: V-set domain containing T cell activation inhibitor 1, VCTN1, PRO1291, B7X, B7S1, B7-H4, B7H4, B7h.5) Copy Product Info

Synonyms: V-set domain containing T cell activation inhibitor 1, VCTN1, PRO1291, B7X, B7S1, B7-H4, B7H4, B7h.5

Catalog No. TMPY-03524 Copy Product Info

B7-H4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.8 kDa and the accession number is Q7Z7D3-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$48	7-10 days	7-10 days
10 µg	$75	-	In Stock
20 µg	$118	-	In Stock
50 µg	$229	-	In Stock
100 µg	$386	-	In Stock
200 µg	$682	7-10 days	7-10 days
500 µg	$1,430	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 97.7%; SEC-HPLC: 99.0%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Immobilized Recombinant Human B7-H4 / B7S1 / B7x Protein (His Tag) at 2 μg/mL (100 μL/well) can bind B7-H4 / B7S1 / B7x, Rabbit Pab , the EC50 is 1.5-4 ng/mL.
Description	B7-H4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.8 kDa and the accession number is Q7Z7D3-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q7Z7D3-1
Construction	A DNA sequence encoding the human VTCN1 (Q7Z7D3-1) (Phe29-Ala258) with a C-terminal polyhistidine tag was expressed. Predicted N terminal: Phe 29
Protein Purity	> 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	V-set domain containing T cell activation inhibitor 1, VCTN1, PRO1291, B7X, B7S1, B7-H4, B7H4, B7h.5
Research Background	V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type-III membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X / VTCN1 / B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X / VTCN1 / B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X / VTCN1 / B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 / interleukin-6 and IL10 / interleukin-10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

26.8 kDa (predicted); 43-48 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.