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EGFR Protein, Human, Recombinant (Isoform vIII, His & Avi), Biotinylated
(Synonyms: PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor) Copy Product InfoSynonyms: PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor
Catalog No. TMPY-06593 Copy Product Info
EGFR Protein, Human, Recombinant (Isoform vIII, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 41.90 kDa and the accession number is NP_001333870.1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $215 | 7-10 days | 7-10 days | |
| 10 μg | $359 | 7-10 days | 7-10 days | |
| 20 μg | $600 | 7-10 days | 7-10 days | |
| 50 μg | $939 | 7-10 days | 7-10 days | |
| 100 μg | $1,580 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Immobilized Anti-EGFR (Cetuximab biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein (Isoform vIII, Avi & His Tag) (Cat#TMPY-06593), the EC50 is 0.5-1.5 ng/mL (QC tested). 2.Immobilized Anti-EGFR (Cetuximab Biosimilar) at 1 μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein (Isoform vIII, Avi & His Tag) (Cat#TMPY-06593), the EC50 is 1.5 ng/mL(Routinely tested). |
| Description | EGFR Protein, Human, Recombinant (Isoform vIII, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 41.90 kDa and the accession number is NP_001333870.1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | His, Avi |
| Accession Number | NP_001333870.1 |
| Construction | A DNA sequence encoding the human EGFR (NP_001333870.1) (Met1-Ser378) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Leu 25 |
| Protein Purity | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor |
| Research Background |
| Molecular Weight | 41.90 kDa (predicted); 64.45 kDa (reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: EGFR Protein, Human, Recombinant (Isoform vIII, His & Avi), Biotinylated chemical structure | EGFR Protein, Human, Recombinant (Isoform vIII, His & Avi), Biotinylated molecular weight
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