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Anti-Phospho-Histone H2AX (Ser139) Antibody (8H231)
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Catalog No. TMAH-00904
Alias Phospho-Histone H2AX (S139), p-Histone H2AX (Ser139), p-Histone H2AX (S139), Histone H2AX (p-Ser139), Histone H2AX (p-S139)
Anti-Phospho-Histone H2AX (Ser139) Antibody (8H231) is an antibody targeting Phospho-Histone H2AX (Ser139). Anti-Phospho-Histone H2AX (Ser139) Antibody (8H231) can be used in ELISA, WB.
Anti-Phospho-Histone H2AX (Ser139) Antibody (8H231)
Anti-Phospho-Histone H2AX (Ser139) Antibody (8H231)
😃Good
Catalog No. TMAH-00904Alias Phospho-Histone H2AX (S139), p-Histone H2AX (Ser139), p-Histone H2AX (S139), Histone H2AX (p-Ser139), Histone H2AX (p-S139)
Anti-Phospho-Histone H2AX (Ser139) Antibody (8H231) is an antibody targeting Phospho-Histone H2AX (Ser139). Anti-Phospho-Histone H2AX (Ser139) Antibody (8H231) can be used in ELISA, WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB Reactivity: Human |
| Alias | Phospho-Histone H2AX (S139), p-Histone H2AX (Ser139), p-Histone H2AX (S139), Histone H2AX (p-Ser139), Histone H2AX (p-S139) |
| Ig Type | Rabbit IgG |
| Clone | 8H231 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in:293 whole cell lysate -All lanes:Phospho-Histone H2AX (S139) antibody at 0.23μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 15 KDa -Observed band size: 15 KDa 2. IHC image of TMAH-00904 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Application | ELISA, WB |
| Recommended Dose | WB:1:3000-1:10000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Chromosome. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide |
| Antigen Species | Human |
| Gene ID | 3014 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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