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Anti-MAX Antibody (6Q204)
😃Good
Catalog No. TMAY-02427
Alias MYC associated factor X, bHLHd4
Anti-MAX Antibody (6Q204) is a Rabbit antibody targeting MAX. Anti-MAX Antibody (6Q204) can be used in WB,ELISA,FCM,ICC/IF,IP.
Anti-MAX Antibody (6Q204)
Anti-MAX Antibody (6Q204)
😃Good
Catalog No. TMAY-02427Alias MYC associated factor X, bHLHd4
Anti-MAX Antibody (6Q204) is a Rabbit antibody targeting MAX. Anti-MAX Antibody (6Q204) can be used in WB,ELISA,FCM,ICC/IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Chemical Properties
| Description | Anti-MAX Antibody (6Q204) is a Rabbit antibody targeting MAX. Anti-MAX Antibody (6Q204) can be used in WB,ELISA,FCM,ICC/IF,IP. |
| Alias | MYC associated factor X, bHLHd4 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6Q204 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human MAX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MAX monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus. 2. Flow cytometric analysis of Human MAX expression on Raji cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. MAX was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate -0.5 µL anti-MAX rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-MAX rabbit monoclonal antibody, at 1:1000 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 23 kDa. -Observed band size: 23 kDa. 4. Anti-MAX rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:18 kDa. -Observed band size:20 kDa(We are unsure as to the identity of these extra bands.) |
| Application | WB,ELISA,FCM,ICC/IF,IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; ICC-IF: 1:100-1:500; FCM: 1:100-1:500; IP: 0.2-1 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human MAX / MYC associated factor X (rh MAX / MYC associated factor X ; TMPY-02888; NP_002373; Met1-Ser160). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| Research Background | MYC associated factor X contains 1 basic helix-loop-helix (bHLH) domain and belongs to the MAX family. It is highly expressed in the brain, heart, and lung while lower levels are seen in the liver, kidney, and skeletal muscle. MYC associated factor X can form homodimers and heterodimers with other family members, which include Mad, Mxi1, and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation, and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. MYC associated factor X may also repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Multiple alternatively spliced transcript variants have been described for MYC associated factor X gene but the full-length nature for some of them is unknown. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human MAX / MYC associated factor X protein (TMPY-02888) |
| Antigen Species | Human |
| Biology Area | Apoptosis Transcription Factors and Regulators |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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