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Anti-ACBD6 Antibody (7G53)
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Catalog No. TMAY-01651
Alias acyl-CoA binding domain containing 6
Anti-ACBD6 Antibody (7G53) is a Mouse antibody targeting ACBD6. Anti-ACBD6 Antibody (7G53) can be used in WB,ELISA,ELISA(Det),IP.
Anti-ACBD6 Antibody (7G53)
Anti-ACBD6 Antibody (7G53)
😃Good
Catalog No. TMAY-01651Alias acyl-CoA binding domain containing 6
Anti-ACBD6 Antibody (7G53) is a Mouse antibody targeting ACBD6. Anti-ACBD6 Antibody (7G53) can be used in WB,ELISA,ELISA(Det),IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $69 | 7-10 days | |
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ELISA, ELISA(Det), IP Reactivity: Human |
| Alias | acyl-CoA binding domain containing 6 |
| Ig Type | Mouse IgG1 |
| Clone | 7G53 |
| Reactivity | Human |
| Verified Activity | 1. ACBD6 was immunoprecipitated using: -Lane A:0.5 mg hela Whole Cell Lysate -0.5 µL anti-ACBD6 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ACBD6 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 31 kDa. -Observed band size: 31 kDa. 2. Anti-ACBD6 mouse monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:31 kDa. -Observed band size:35 kDa |
| Application | WB,ELISA,ELISA(Det),IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000; IP: 1-2 μL/mg of lysate; ELISA(Det): 1:5000-1:50000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACBD6 (rh ACBD6; TMPY-01345; NP_115736.1; Met 1-Ala 282). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography . |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Human acyl-coenzyme A binding domain-containing member 6 (ACBD6) is a modular protein that carries an acyl-CoA binding domain at its N terminus and two ankyrin motifs at its C terminus. In mammals, there are six members of the acyl-CoA binding domain-containing (ACBD) family, and their annotation is not uniform. All six ACBD proteins contain an ACB domain at the N terminus, but they do not share significant homology at the C-terminal region. ACBD6 is a 32 kDa protein that is predicted by sequence analysis to carry an ACB domain between residues 42 and 125 and two ANK motifs at its C terminus. This protein binds long-chain acyl-CoAs with a strong preference for unsaturated, C18:1-CoA and C20:4-CoA, over saturated, C16:0-CoA, acyl species. ACBD6 is not a ubiquitous protein, but it is expressed in hematopoietic tissues and appears to be restricted to primitive stem cells present in those tissues with functions in blood and vessel development. ACBD6 was detected in bone marrow, spleen, placenta, cord blood, circulating CD34+ progenitors, and embryonic-like stem cells derived from placenta. In placenta, the protein was only detected in CD34+ progenitor cells present in blood and CD31+ endothelial cells surrounding the blood vessels. These cells were also positive for the marker CD133, and they probably constitute hemangiogenic stem cells, precursors of both blood and vessels. We propose that human ACBD6 represents a cellular marker for primitive progenitor cells with functions in hematopoiesis and vascular endothelium development. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ACBD6 protein (TMPY-01345) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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