CD64 Protein, Mouse, Recombinant (His)

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

Pack Size	Availability	Price/USD
100 μg	In stock	$ 386.00
200 μg	5 days	$ 682.00
500 μg	5 days	$ 1,440.00

Biological Information

1. Immobilized Rituximab (IgG1) at 1 μg/mL can bind Mouse FcγRI / CD64 recombinant protein, the EC50 is 16-35 ng/mL. 2. Immobilized Anti-CD3 antibody, mouse IgG2a at 2 μg/mL (100 μL/well) can bind Recombinant Mouse CD64 / FCGR1 Protein (His Tag) , the EC50 is 0.8-2.4 ng/mL(QC tested). 3.Loaded Mouse FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in a BLI assay. 4.Loaded Mouse FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 0.28 µM as determined in a BLI assay. 5.Captured Mouse FcγRI / CD64 recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in an SPR assay. 6.Captured Mouse FcγRI / CD64 recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.69 µM as determined in an SPR assay (Biacore T200). 7. The purity of Mouse CD64 / FCGR1 Protein (His Tag) is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS. 8.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a on ProA Biosensor, can bind Recombinant Mouse CD64 Protein, His Tag with an affinity constant of 3.46nM as determined in BLI assay(QC tested).

Description

Species	Mouse
Expression System	HEK293
Tag	His
Accession Number	P26151
Synonyms	Fc receptor, IgG, high affinity I, IGGHAFC, AI323638, FcgammaRI, CD64, FcγRI, AV092959
Construction	A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) precursor was fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Molecular Weight	Approxiamtely 32 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.