Siglec-2/CD22 Protein, Human, Recombinant (hFc)

Name: Siglec-2/CD22 Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPJ-00393
Rating: 4.5 (1 reviews)

(Synonyms: T-cell surface antigen Leu-14, SIGLEC2FLJ22814, Siglec-2, sialic acid binding Ig-like lectin 2, CD22 molecule, CD22 antigenMGC130020, CD22, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22) Copy Product Info

Synonyms: T-cell surface antigen Leu-14, SIGLEC2FLJ22814, Siglec-2, sialic acid binding Ig-like lectin 2, CD22 molecule, CD22 antigenMGC130020, CD22, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22

Catalog No. TMPJ-00393 Copy Product Info

Siglec-2/CD22 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 120-150 KDa and the accession number is P20273.

Pack Size	Price	USA Stock	Global Stock
5 µg	$80	35 days	35 days
10 µg	$129	35 days	35 days
20 µg	$198	35 days	35 days
50 µg	$390	35 days	35 days
100 µg	$626	35 days	35 days
200 µg	$987	35 days	35 days
500 µg	$1,900	35 days	35 days
1 mg	$2,730	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Resource Download

User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Anti-Human Siglec-2 mAb at 2μg/ml (100 μl/well)can bind Human Siglec-2-Fc. The ED50 of Human Siglec-2-Fc is 24.05 ng/ml. (Regularly tested)
Description	Siglec-2/CD22 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 120-150 KDa and the accession number is P20273.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P20273
Amino Acid	Asp20-Arg687
Construction	Asp20-Arg687
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	T-cell surface antigen Leu-14, SIGLEC2FLJ22814, Siglec-2, sialic acid binding Ig-like lectin 2, CD22 molecule, CD22 antigenMGC130020, CD22, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22
Research Background	Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.

Chemical Properties

Molecular Weight

120-150 KDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.