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Anti-Phospho-SMAD5 (Ser463, 465) Antibody (3D336) is an antibody targeting Phospho-SMAD5 (Ser463, 465). Anti-Phospho-SMAD5 (Ser463, 465) Antibody (3D336) can be used in ELISA,IHC.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $208 | 7-10 days | 7-10 days | |
| 100 μL | $347 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-SMAD5 (Ser463, 465) Antibody (3D336) is an antibody targeting Phospho-SMAD5 (Ser463, 465). Anti-Phospho-SMAD5 (Ser463, 465) Antibody (3D336) can be used in ELISA,IHC. |
| Synonyms | SMAD5 (p-Ser463, 465), SMAD5 (p-S463, 465), SMAD family member 5, SMAD 5, SMAD, SMA and MAD related protein 5, p-SMAD5 (Ser463, 465), p-SMAD5 (S463, 465), Phospho-SMAD5 (S463, 465), MusMLP, mothers against DPP homolog 5, mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD homolog 5, MAD, JV5 1, hSmad5, Dwfc, DKFZp781O1323, DKFZp781C1895 |
| Ig Type | Rabbit IgG |
| Clone | 3D336 |
| Reactivity | Human |
| Verified Activity | IHC image of TMAH-00966 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Application | |
| Recommended Dose | IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. |
| Tissue Specificity | Ubiquitous. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-SMAD5 (S463+S465) |
| Antigen Species | Human |
| Gene Name | SMAD5 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Signal Transduction |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
| Size | Quantity | Unit Price | Amount | Operation |
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