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Anti-Phospho-SMAD5 (Ser463, 465) Antibody (3D336) is an antibody targeting Phospho-SMAD5 (Ser463, 465). Anti-Phospho-SMAD5 (Ser463, 465) Antibody (3D336) can be used in ELISA, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Anti-Phospho-SMAD5 (Ser463, 465) Antibody (3D336) is an antibody targeting Phospho-SMAD5 (Ser463, 465). Anti-Phospho-SMAD5 (Ser463, 465) Antibody (3D336) can be used in ELISA, IHC. |
Alias | SMAD family member 5, SMAD 5, SMAD, SMA and MAD related protein 5, Phospho-SMAD5 (S463, 465), MusMLP, Mothers against DPP homolog 5, mothers against DPP homolog 5, mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD homolog 5, MAD, JV5 1, hSmad5, Dwfc, DKFZp781O1323, DKFZp781C1895 |
Ig Type | Rabbit IgG |
Clone | 3D336 |
Reactivity | Human |
Verified Activity | IHC image of TMAH-00966 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Application | ELISA, IHC |
Recommended Dose | IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human Phospho-SMAD5 (S463+S465) |
Antigen Species | Human |
Gene ID | 4090 |
Uniprot ID | |
Biology Area | Signal Transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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