Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, ICC, FCM

Reactivity: Human, Mouse

Rabbit IgG

2G840

Human, Mouse

1. Western Blot
-Positive WB detected in:Hela whole cell lysate,293 whole cell lysate,NIH/3T3 whole cell lysate
-All lanes: Phospho-Histone H3 (T3) antibody at 1.41μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 16 KDa
-Observed band size: 16 KDa
2. Immunocytochemistry analysis of TMAH-00908 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. Overlay histogram showing Hela cells stained with TMAH-00908 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, ICC, FCM

WB:1:500-1:5000; ICC:1:50-1:500.

Monoclonal

Unconjugated

A synthetic peptide

Human

3020

Epigenetics and Nuclear Signaling