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Anti-Secretin Polyclonal Antibody

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Catalog No. TMAB-12627

Anti-Secretin Polyclonal Antibody is a Rabbit antibody targeting Secretin. Anti-Secretin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-Secretin Polyclonal Antibody

Anti-Secretin Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-12627
Anti-Secretin Polyclonal Antibody is a Rabbit antibody targeting Secretin. Anti-Secretin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Secretin Polyclonal Antibody is a Rabbit antibody targeting Secretin. Anti-Secretin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse)
Verified Activity
1. Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Secrein Polyclonal Antibody, Unconjugated (TMAB-12627) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 11: Human SW480 cell lysates
Lane 12: Human MCF-7 cell lysates
Lane 13: Human HepG2 cell lysates
Primary: Anti-Secretin (TMAB-12627) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research Backgroundbs-0088P is one synthetic peptide derived from human Secretin. Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Secretin
Antigen Species
Human
Gene Name
Sct
Gene ID
Protein Name
Secretin
Function
Stimulates formation of NaHCO(3)-rich pancreatic juice and secretion of NaHCO(3)-rich bile and inhibits HCl production by the stomach.
Chemical Properties
Molecular WeightTheoretical: 13 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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