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Anti-GRIN1 Antibody (5S823) is an antibody targeting GRIN1. Anti-GRIN1 Antibody (5S823) can be used in ELISA, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, FCM Reactivity: Human |
Ig Type | Rabbit IgG |
Clone | 5S823 |
Reactivity | Human |
Verified Activity | Overlay Peak curve showing SH-SY5Y cells stained with TMAH-00515 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, FCM |
Recommended Dose | FCM:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic density. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human GRIN1 |
Antigen Species | Human |
Gene ID | 2902 |
Uniprot ID | |
Biology Area | Neuroscience |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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