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Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (8L659)
🥰Excellent
Catalog No. TMAY-02877
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (8L659) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (8L659) can be used in WB,ELISA,IHC-P,FCM,ICC/IF.
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (8L659)
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (8L659)
🥰Excellent
Catalog No. TMAY-02877
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (8L659) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (8L659) can be used in WB,ELISA,IHC-P,FCM,ICC/IF.
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Related Conjugates and Formulations
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Chemical Properties
| Description | Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (8L659) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (8L659) can be used in WB,ELISA,IHC-P,FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit (variable region) / human (kappa / IgG1 constant) chimeric antibody |
| Clone | 8L659 |
| Verified Activity | 1. Flow cytometric analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Nucleocapsid Chimeric MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. (Validation Experiment) 2. Anti-SARS-CoV / SARS-CoV-2 Nucleocapsid Chimeric monoclonal antibody at 1:2000 dilution. -Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate. -Lane B: HEK293 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Human IgG Fc/HRP at 1/1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. (Validation Experiment) 3. Immunochemical analysis of SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a HRP-conjugated second step antibody. (Validation Experiment) 4. Immunofluorescence analysis of SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a FITC-conjugated second step antibody. (Validation Experiment) |
| Application | WB,ELISA,IHC-P,FCM,ICC/IF |
| Recommended Dose | WB: 1:2000-1:5000; ELISA: 1:5000-1:10000; IHC-P: 1:20-1:100; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Chimera |
| Construction | It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with rabbit variable regions, and the variable region was from rabbit monoclonal antibody (Cat#40143-R004). The chimeric monoclonal antibody was produced using recombinant antibody technology. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant SARS-CoV Nucleoprotein / NP Protein (TMPY-02870) |
| Antigen Species | SARS |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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