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Anti-Histone H2A type 1-B/E Antibody (2D761) is an antibody targeting Histone H2A type 1-B/E. Anti-Histone H2A type 1-B/E Antibody (2D761) can be used in ELISA, WB, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, IF Reactivity: Human |
Alias | MGC74460, Histone H2A/m, Histone H2A/a, Histone H2A.2, Histone H2A type 1 J, Histone H2A type 1 E, Histone H2A type 1 C, Histone H2A type 1 B, Histone 2 H2ac, Histone 2 H2ab, Histone 2 H2aa3, Histone 1 H2ae, HIST2H2AC, HIST2H2AB, HIST2H2AA3, HIST2H2AA, HIST1H2AJ, HIST1H2AE, H2AFQ, H2AFO, H2AFM, H2AFL, H2AFE, H2AFA, H2A1B_HUMAN, H2A/q, H2A/O, H2A/m, H2A/a, H2A.2, H2A.1, H2A histone family member A, H2A GL101, H2a 615, H2A |
Ig Type | Rabbit IgG |
Clone | 2D761 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in HepG2 whole cell lysate -All lanes Histone H2A type 1-B/E antibody at 2.7μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 13 KDa -Observed band size: 13 KDa 2. Immunofluorescence staining of Hela cells with TMAH-00551 at 1:168,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, WB, IF |
Recommended Dose | WB:1:500-1:5000; IF:1:30-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Nucleus. Chromosome. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide |
Antigen Species | Human |
Gene ID | 3012 |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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