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Anti-IL-23 Polyclonal Antibody
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Catalog No. TMAB-00956
Anti-IL-23 Polyclonal Antibody is a Rabbit antibody targeting IL-23. Anti-IL-23 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-IL-23 Polyclonal Antibody
Anti-IL-23 Polyclonal Antibody
😃Good
Catalog No. TMAB-00956
Anti-IL-23 Polyclonal Antibody is a Rabbit antibody targeting IL-23. Anti-IL-23 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-IL-23 Polyclonal Antibody is a Rabbit antibody targeting IL-23. Anti-IL-23 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Tissue/cell: human rhinopolypus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-IL-23 Polyclonal Antibody, Unconjugated (TMAB-00956) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Mouse Ear tissue lysates Lane 2: Human A431 cell lysates Lane 3: Human Jurkat cell lysates Primary: Anti-IL-23 (TMAB-00956) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. Secreted upon association with IL12B. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | IL-23 is a heterodimeric cytokine composed of two subunits: a unique p19 subunit, and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated dendritic cells and macrophages. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T cells and promotes production of proinflammatory cytokines, such as IFNγ and IL-17. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. This recombinant human IL-23 is produced by human cells.(Tag free) Biological activity: The activity was measured by its ability to induce the IL-17 expression in murine splenocytes. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| Immunogen | KLH conjugated synthetic peptide: human IL-23 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Interleukins,Interleukins |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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