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Anti-TROP-2 Antibody (6N25)

😃Good
Catalog No. TMAY-00815
Alias tumor-associated calcium signal transducer 2, TROP-2, TROP2, TACD2, M1S1, GP50, GA733-1, GA7331, EGP-1, EGP1

Anti-TROP-2 Antibody (6N25) is a Mouse antibody targeting TROP-2. Anti-TROP-2 Antibody (6N25) can be used in ELISA(Cap),IHC-P,ICC/IF.

Anti-TROP-2 Antibody (6N25)

Anti-TROP-2 Antibody (6N25)

😃Good
Catalog No. TMAY-00815Alias tumor-associated calcium signal transducer 2, TROP-2, TROP2, TACD2, M1S1, GP50, GA733-1, GA7331, EGP-1, EGP1
Anti-TROP-2 Antibody (6N25) is a Mouse antibody targeting TROP-2. Anti-TROP-2 Antibody (6N25) can be used in ELISA(Cap),IHC-P,ICC/IF.
Pack SizePriceAvailabilityQuantity
20 μL$70 7-10 days
100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-TROP-2 Antibody (6N25) is a Mouse antibody targeting TROP-2. Anti-TROP-2 Antibody (6N25) can be used in ELISA(Cap),IHC-P,ICC/IF.
Aliastumor-associated calcium signal transducer 2, TROP-2, TROP2, TACD2, M1S1, GP50, GA733-1, GA7331, EGP-1, EGP1
Ig Type
Monoclonal Mouse IgG1
Clone
6N25
Reactivity
Human, Rhesus, Cynomolgus;
Not React with: Mouse, Rat
Verified Activity
1. Immunofluorescence staining of human TROP2 in MCF-7 cells with mouse monoclonal antibody (1:60). The image showing membrane staining of MCF-7 cells.

2. Immunochemical staining of human TROP2 in human rectal cancer with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

3. Immunochemical staining of human TROP2 in human placenta with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of cytotrophoblast.

4. Immunochemical staining of human TROP2 in human mammary gland with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of alveolus epithelium.

5. Immunofluorescence staining of TROP-2 in NCI-N87 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with mouse anti-Human TROP-2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane.
Application
ELISA(Cap),IHC-P,ICC/IF
Recommended Dose
IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100; ELISA(Cap): 1:250-1:2000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TROP2 extracellular domain (rh TROP2; TMPY-01262; NP_002344.2; Met 1-Thr 274). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundTROP-2, also referred to as tumor-associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, which are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human TROP2 protein (TMPY-01262)
Antigen Species
Human
Biology Area
Cancer Drug Targets
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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