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ROBO4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.8 kDa and the accession number is Q8WZ75-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $600 | 7-10 days |
Biological Activity | Immobilized Human ROBO4, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8 ng/ml determined by ELISA. |
Description | ROBO4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.8 kDa and the accession number is Q8WZ75-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | Q8WZ75-1 |
Synonyms | roundabout, axon guidance receptor, homolog 4 (Drosophila),MRB,ECSM4 |
Construction | Ala27-Glu469 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 47.8 kDa (Predicted); 55-70 kDa (Due to glycosylation) |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis. |
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