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Synonyms: roundabout, axon guidance receptor, homolog 4 (Drosophila), MRB, ECSM4

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $70 | - | In Stock | |
| 10 µg | $109 | - | In Stock | |
| 20 µg | $182 | - | In Stock | |
| 50 µg | $357 | - | In Stock | |
| 100 µg | $600 | - | In Stock |
| Bioactivity | Immobilized Human ROBO4, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8 ng/ml determined by ELISA. |
| Description | ROBO4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.8 kDa and the accession number is Q8WZ75-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | Q8WZ75-1 |
| Construction | Ala27-Glu469 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | roundabout, axon guidance receptor, homolog 4 (Drosophila), MRB, ECSM4 |
| Research Background | Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis. |
| Molecular Weight | 47.8 kDa (Predicted); 55-70 kDa (Due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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