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VEGF121b Protein, Human, Recombinant
(Synonyms: VPF, VEGF, vascular endothelial growth factor A, MVCD1) Copy Product InfoSynonyms: VPF, VEGF, vascular endothelial growth factor A, MVCD1
Catalog No. TMPY-03698 Copy Product Info
Purity: 97.7%
VEGF121b Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 34.10 kDa and the accession number is P15692-9.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $59 | - | In Stock | |
| 10 μg | $93 | - | In Stock | |
| 20 μg | $152 | - | In Stock | |
| 50 μg | $296 | - | In Stock | |
| 100 μg | $507 | 7-10 days | 7-10 days | |
| 200 μg | $865 | 7-10 days | 7-10 days | |
| 500 μg | $1,750 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:97.7%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Measured by its binding ability in a functional ELISA. Immobilized human VEGF121b at 10 μg/mL (100μL/well) can bind human VEGFR2-Fc, the EC50 of human VEGFR2-Fc is 0.06-0.1 μg/mL.
2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 1-5 ng/mL.   |
| Description | VEGF121b Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 34.10 kDa and the accession number is P15692-9. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | Tag Free |
| Accession Number | P15692-9 |
| Construction | A DNA sequence encoding the human VEGF 121 isoform b (P15692-9) (Met1-Asp147,with last 6 amino acids switch from CDKPRR to SLTRKD ) was expressed. Predicted N terminal: Ala 27 |
| Protein Purity | > 95 % as determined by SDS-PAGE  |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | VPF, VEGF, vascular endothelial growth factor A, MVCD1 |
| Research Background |
| Molecular Weight | 34.10 kDa (predicted); 20 and 16 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: VEGF121b Protein, Human, Recombinant chemical structure | VEGF121b Protein, Human, Recombinant molecular weight
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