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Anti-Chorionic Gonadotropin/CGA Antibody (3J474)

(Synonyms: TSHA, LHA, HCG, GPHA1, GPHa, glycoprotein hormones, α polypeptide, glycoprotein hormones, alpha polypeptide, FSHA, FSH α, FSH alpha, CG-α, CG-ALPHA) Copy Product Info
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Synonyms: TSHA, LHA, HCG, GPHA1, GPHa, glycoprotein hormones, α polypeptide, glycoprotein hormones, alpha polypeptide, FSHA, FSH α, FSH alpha, CG-α, CG-ALPHA

Catalog No. TMAB-00428 Copy Product Info
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Anti-Chorionic Gonadotropin/CGA Antibody (3J474) is a Mouse antibody targeting Chorionic Gonadotropin/CGA. Anti-Chorionic Gonadotropin/CGA Antibody (3J474) can be used in ELISA,IF,IHC-Fr,IHC-P.

Anti-Chorionic Gonadotropin/CGA Antibody
(3J474)

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2217-10 days7-10 days
100 µL$3747-10 days7-10 days
200 µL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Chorionic Gonadotropin/CGA Antibody (3J474) is a Mouse antibody targeting Chorionic Gonadotropin/CGA. Anti-Chorionic Gonadotropin/CGA Antibody (3J474) can be used in ELISA,IF,IHC-Fr,IHC-P.
SynonymsTSHA, LHA, HCG, GPHA1, GPHa, glycoprotein hormones, α polypeptide, glycoprotein hormones, alpha polypeptide, FSHA, FSH α, FSH alpha, CG-α, CG-ALPHA
Ig Type
IgG
Clone
3J474
Reactivity
Human
Verified Activity
1. Measured by its binding ability in a indirect ELISA. Immobilized Human HCG alpha protein, HisTag at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human HCG alpha Antibody, the EC50 is 40.93 ng/mL.
2. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated (TMAB-00428) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human pituitary gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated (TMAB-00428) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated (TMAB-00428) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Anti-Chorionic Gonadotropin/CGA Antibody (3J474),Anti-Chorionic Gonadotropin/CGA Antibody (3J474),Anti-Chorionic Gonadotropin/CGA Antibody (3J474),Anti-Chorionic Gonadotropin/CGA Antibody (3J474)
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationSecreted.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M PBS (pH7.4) with 0.02% Proclin300.
Concentration1 mg/mL
Research BackgroundThe four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Antigen Details
Immunogen
Natural human hCG protein
Antigen Species
Human
Gene Name
CGA
Gene ID
Protein Name
Glycoprotein hormones alpha chain
Uniprot ID
Biology Area
Hormone biosynthesis,Hormone biosynthesis,Cancer,Gonadotrophic axis,Thyroid axis,Hormones
Function
Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Chemical Properties
Molecular WeightTheoretical: 10 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: Anti-Chorionic Gonadotropin/CGA Antibody (3J474) molecular weight