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Anti-Chorionic Gonadotropin/CGA Antibody (3J474)

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Catalog No. TMAB-00428

Anti-Chorionic Gonadotropin/CGA Antibody (3J474) is a Mouse antibody targeting Chorionic Gonadotropin/CGA. Anti-Chorionic Gonadotropin/CGA Antibody (3J474) can be used in IHC-P,IHC-Fr,IF,ELISA.

Anti-Chorionic Gonadotropin/CGA Antibody (3J474)

Anti-Chorionic Gonadotropin/CGA Antibody (3J474)

😃Good
Catalog No. TMAB-00428
Anti-Chorionic Gonadotropin/CGA Antibody (3J474) is a Mouse antibody targeting Chorionic Gonadotropin/CGA. Anti-Chorionic Gonadotropin/CGA Antibody (3J474) can be used in IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Mouse Monoclonal

Application: IHC-P,IHC-Fr,IF,ELISA

Reactivity: Human
Ig Type
IgG
Clone
3J474
Reactivity
Human
Verified Activity
1. Measured by its binding ability in a indirect ELISA. Immobilized Human HCG alpha protein, HisTag at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human HCG alpha Antibody, the EC50 is 40.93 ng/mL.
2. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated (TMAB-00428) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human pituitary gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated (TMAB-00428) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated (TMAB-00428) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Application
IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationSecreted.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M PBS (pH7.4) with 0.02% Proclin300.
Research BackgroundThe four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Antigen Details
Immunogen
Natural human hCG protein
Antigen Species
Human
Gene ID
1081
Uniprot ID
Biology Area
Hormone biosynthesis,Hormone biosynthesis,Cancer,Gonadotrophic axis,Thyroid axis,Hormones
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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