Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, ICC, IF

Reactivity: Human

Rabbit IgG

2A639

Human

1. Western Blot
-Positive WB detected in Hela whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate
-All lanes Mono-methyl-Histone H3.1(R2)antibody at 0.95μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 15 KDa
-Observed band size: 15 KDa
2. Immunocytochemistry analysis of TMAH-00776 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. Immunofluorescence staining of Hela cells with TMAH-00776 at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ELISA, WB, ICC, IF

WB:1:500-1:2000; ICC:1:50-1:500; IF:1:30-1:200.

Monoclonal

Unconjugated

A synthetic peptide

Human

8350

Epigenetics and Nuclear Signaling