Cripto Protein, Human, Recombinant (hFc)

Name: Cripto Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-05503
Rating: 4.5 (1 reviews)

(Synonyms: teratocarcinoma-derived growth factor 1, CRIPTO, CRGF, CR) Copy Product Info

Synonyms: teratocarcinoma-derived growth factor 1, CRIPTO, CRGF, CR

Catalog No. TMPY-05503 Copy Product Info

Cripto Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.7 kDa and the accession number is P13385.

Pack Size	Price	USA Stock	Global Stock
5 µg	$87	7-10 days	7-10 days
10 µg	$142	7-10 days	7-10 days
20 µg	$233	7-10 days	7-10 days
50 µg	$535	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Resource Download

User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized ALK4-His at 10 μg/mL (100 μL/well) can bind TDGF1-Fc , the EC50 of human TDGF1-Fc is 6.0-14 ng/mL.
Description	Cripto Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.7 kDa and the accession number is P13385.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P13385
Construction	A DNA sequence encoding the human TDGF1 (NP_003203.1) (Met1-Thr172) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Leu 31
Protein Purity	> 95 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	teratocarcinoma-derived growth factor 1, CRIPTO, CRGF, CR
Research Background	Cripto/TDGF1 is a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family. EGF-CFC family member proteins share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. Before gastrulation, Cripto is asymmetrically expressed in a proximal–distal gradient in the epiblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm. These proteins play key roles in intercellular signaling pathways during vertebrate embryogenesis. Mutations in Cripto/TDGF1 can cause autosomal visceral heterotaxy. Cripto/TDGF1 is involved in left-right asymmetric morphogenesis during organ development. Cripto signalling is essential for the conversion of a proximal–distal asymmetry into an orthogonal anterior–posterior axis. The mechanism of inhibitory effects of the Cripto includes both cancer cell apoptosis, activation of c-Jun-NH(2)-terminal kinase and p38 kinase signaling pathways and blocking of Akt phosphorylation. Thus, Cripto is a unique target, and Immunohistochemistry to Cripto could be of therapeutic value for human cancers.

Chemical Properties

Molecular Weight

42.7 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.