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GUCY2C Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.78 kDa and the accession number is P25092-1.

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $68 | 7-10 days | 7-10 days | |
| 10 μg | $108 | 7-10 days | 7-10 days | |
| 20 μg | $178 | 7-10 days | 7-10 days | |
| 50 μg | $359 | 7-10 days | 7-10 days | |
| 100 μg | $696 | 7-10 days | 7-10 days | |
| 200 μg | $1,060 | 7-10 days | 7-10 days | |
| 500 μg | $2,230 | 7-10 days | 7-10 days | |
| 1 mg | $4,530 | 7-10 days | 7-10 days |
| Biological Activity | 1.Loaded anti-GUCY2C/Guanylyl cyclase C scFv-hIgG1 Fc on proA Biosensor, can bind Recombinant Human GUCY2C/Guanylyl cyclase C Protein, His Tag (Cat#TMPY-06516) with an affinity constant of 6.440 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 2.Immobilized Recombinant Human GUCY2C Protein (His Tag) (Cat#TMPY-06516) at 5 μg/mL (100 μL/well) can bind anti-GUCY2C Guanylyl cyclase C Antibody, the EC50 is 2-6 ng/mL (QC tested). |
| Description | GUCY2C Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.78 kDa and the accession number is P25092-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P25092-1 |
| Synonyms | STAR,MUCIL,MECIL,GUC2C,DIAR6 |
| Construction | A DNA sequence encoding the human GUCY2C (P25092-1) (Met1-Gln430) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Ser 24 |
| Protein Purity | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. |
| Molecular Weight | 47.78 kDa (predicted); 72.11 kDa (reducing conditions) |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
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