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PLAU/uPA Protein (active form), Human, Recombinant (His & Avi), Biotinylated
(Synonyms: Urokinase, URK, u-PA, UPA, QPD, PLAU, BDPLT5, ATF) Copy Product InfoSynonyms: Urokinase, URK, u-PA, UPA, QPD, PLAU, BDPLT5, ATF
Catalog No. TMPK-00274 Copy Product Info
Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC. PLAU/uPA Protein (active form), Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 49.3 kDa and the accession number is P00749-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $91 | - | In Stock | |
| 10 µg | $148 | - | In Stock | |
| 20 µg | $245 | 7-10 days | 7-10 days | |
| 50 µg | $483 | - | In Stock | |
| 100 µg | $813 | - | In Stock | |
| 200 µg | $1,470 | 7-10 days | 7-10 days | |
| 500 µg | $3,250 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Liquid
Color:Transparent
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human uPAR, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.6ng/ml determined by ELISA. |
| Description | Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC. PLAU/uPA Protein (active form), Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 49.3 kDa and the accession number is P00749-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P00749-1 |
| Construction | Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-Terminus fragment (Ser21-Lys155). |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | Urokinase, URK, u-PA, UPA, QPD, PLAU, BDPLT5, ATF |
| Research Background | Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC. |
| Molecular Weight | 49.3 kDa (predicted). Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa(long chain A), 35-38 kDa(chain B) and 52-60 kDa(long chain A&chain B) based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: PLAU/uPA Protein (active form), Human, Recombinant (His & Avi), Biotinylated chemical structure | PLAU/uPA Protein (active form), Human, Recombinant (His & Avi), Biotinylated molecular weight
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