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Anti-His Tag Polyclonal Antibody
😃Good
Catalog No. TMAB-00863
Alias HisX6, Histidine, His-Tag, HHHHHH tag, HHHHHH epitope tag, Hexa His tag, 6His, 6 His epitope tag
Anti-His Tag Polyclonal Antibody is a Rabbit antibody targeting His Tag. Anti-His Tag Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
Anti-His Tag Polyclonal Antibody
Anti-His Tag Polyclonal Antibody
😃Good
Catalog No. TMAB-00863Alias HisX6, Histidine, His-Tag, HHHHHH tag, HHHHHH epitope tag, Hexa His tag, 6His, 6 His epitope tag
Anti-His Tag Polyclonal Antibody is a Rabbit antibody targeting His Tag. Anti-His Tag Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μL | $107 | 7-10 days | 7-10 days | |
| 200 μL | $184 | 7-10 days | 7-10 days | |
| 500 μL | $427 | 7-10 days | 7-10 days | |
| 1 mL | $804 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-His Tag Polyclonal Antibody is a Rabbit antibody targeting His Tag. Anti-His Tag Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB. |
| Synonyms | HisX6, Histidine, His-Tag, HHHHHH tag, HHHHHH epitope tag, Hexa His tag, 6His, 6 His epitope tag |
| Ig Type | IgG |
| Reactivity | Species independent |
| Verified Activity | 20 ng rGFP-His protein per Lane probed with His tag polyclonal antibody respectively, unconjugated (TMAB-00863) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at RT for 60 min.  |
| Application | |
| Recommended Dose | ELISA=1:1000-5000; FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Immunogen | Synthetic peptide: HHHHHH conjugated to KLH |
| Protein Name | His tag |
| Biology Area | 6x his tag®,His Tag |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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