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Anti-LYVE1 Antibody (5P861)
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Catalog No. TMAH-00716
Alias XLKD1, LYVE-1, Lymphatic vessel endothelial hyaluronic acid receptor 1, Hyaluronic acid receptor, HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1
Anti-LYVE1 Antibody (5P861) is an antibody targeting LYVE1. Anti-LYVE1 Antibody (5P861) can be used in ELISA, IF, FCM.
Anti-LYVE1 Antibody (5P861)
Anti-LYVE1 Antibody (5P861)
😃Good
Catalog No. TMAH-00716Alias XLKD1, LYVE-1, Lymphatic vessel endothelial hyaluronic acid receptor 1, Hyaluronic acid receptor, HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1
Anti-LYVE1 Antibody (5P861) is an antibody targeting LYVE1. Anti-LYVE1 Antibody (5P861) can be used in ELISA, IF, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IF, FCM Reactivity: Human |
| Alias | XLKD1, LYVE-1, Lymphatic vessel endothelial hyaluronic acid receptor 1, Hyaluronic acid receptor, HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1 |
| Ig Type | Rabbit IgG |
| Clone | 5P861 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of HepG2 with TMAH-00716 at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 505-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing HepG2 cells surface stained with TMAH-00716 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, IF, FCM |
| Recommended Dose | IF:1:50-1:200; FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. Note=Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human LYVE1 |
| Antigen Species | Human |
| Gene ID | 10894 |
| Uniprot ID | |
| Biology Area | Cardiovascular |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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