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Anti-SOD1 Antibody (7K225)

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Catalog No. TMAH-01120

Anti-SOD1 Antibody (7K225) is an antibody targeting SOD1. Anti-SOD1 Antibody (7K225) can be used in ELISA, WB, FCM.

Anti-SOD1 Antibody (7K225)

Anti-SOD1 Antibody (7K225)

😃Good
Catalog No. TMAH-01120
Anti-SOD1 Antibody (7K225) is an antibody targeting SOD1. Anti-SOD1 Antibody (7K225) can be used in ELISA, WB, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$207 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, FCM

Reactivity: Human
Ig Type
Rabbit IgG
Clone
7K225
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate
-All lanes: SOD1 antibody at 1:1500
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 16 kDa
-Observed band size: 18 kDa
2. Overlay histogram showing Jurkat cells stained with TMAH-01120 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, WB, FCM
Recommended Dose
WB:1:500-1:5000; FCM:1:20-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCytoplasm. Mitochondrion. Nucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundDestroys radicals which are normally produced within the cells and which are toxic to biological systems.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human SOD1
Antigen Species
Human
Gene ID
6647
Uniprot ID
Biology Area
Cancer, Cell biology, Metabolism, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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