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Anti-SOD1 Antibody (7K225) is an antibody targeting SOD1. Anti-SOD1 Antibody (7K225) can be used in ELISA, WB, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, FCM Reactivity: Human |
Ig Type | Rabbit IgG |
Clone | 7K225 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate -All lanes: SOD1 antibody at 1:1500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 16 kDa -Observed band size: 18 kDa 2. Overlay histogram showing Jurkat cells stained with TMAH-01120 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, WB, FCM |
Recommended Dose | WB:1:500-1:5000; FCM:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cytoplasm. Mitochondrion. Nucleus. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Destroys radicals which are normally produced within the cells and which are toxic to biological systems. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human SOD1 |
Antigen Species | Human |
Gene ID | 6647 |
Uniprot ID | |
Biology Area | Cancer, Cell biology, Metabolism, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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