Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human

Rabbit IgG

7U863

Human

1. Western Blot
-Positive WB detected in: PC-3 whole cell lysate, THP-1 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, U87 whole cell lysate
-All lanes: PTPN1 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 50 kDa
-Observed band size: 50 kDa
2. IHC image of TMAH-01021 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-01021 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

ELISA, WB, IHC

WB:1:500-1:5000; IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human PTP1B

Human

5770

Cardiovascular, Signal transduction