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Anti-CXADR/CAR Antibody (7D440)
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Catalog No. TMAY-01175
Alias HCAR, CXADR, coxsackie virus and adenovirus receptor, CAR4/6, CAR
Anti-CXADR/CAR Antibody (7D440) is a Rabbit antibody targeting CXADR/CAR. Anti-CXADR/CAR Antibody (7D440) can be used in ELISA(Det),FCM,ICC/IF.
Anti-CXADR/CAR Antibody (7D440)
Anti-CXADR/CAR Antibody (7D440)
😃Good
Catalog No. TMAY-01175Alias HCAR, CXADR, coxsackie virus and adenovirus receptor, CAR4/6, CAR
Anti-CXADR/CAR Antibody (7D440) is a Rabbit antibody targeting CXADR/CAR. Anti-CXADR/CAR Antibody (7D440) can be used in ELISA(Det),FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $270 | 7-10 days |
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Bioactivity
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Antigen Details
Chemical Properties
| Description | Anti-CXADR/CAR Antibody (7D440) is a Rabbit antibody targeting CXADR/CAR. Anti-CXADR/CAR Antibody (7D440) can be used in ELISA(Det),FCM,ICC/IF. |
| Alias | HCAR, CXADR, coxsackie virus and adenovirus receptor, CAR4/6, CAR |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 7D440 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane. 2. Flow cytometric analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA(Det),FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human CXADR / CAR (rh CXADR / CAR; TMPY-02121; NP_001329.1; Met1-Gly237). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| Research Background | CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR. |
| Conjucates | Unconjugated |
| Others Formats | FITC/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human CXADR / CAR protein (TMPY-02121) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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