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Anti-MMP-9 Antibody (5B504) is an antibody targeting MMP-9. Anti-MMP-9 Antibody (5B504) can be used in ELISA, IHC, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IHC, FCM Reactivity: Human |
Alias | MMP-9, MANDP2, GELB, Gelatinase B, CLG4B |
Ig Type | Rabbit IgG |
Clone | 5B504 |
Reactivity | Human |
Verified Activity | 1. IHC image of TMAH-00765 diluted at 1:235 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 2. Overlay histogram showing Jurkat cells stained with TMAH-00765 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, IHC, FCM |
Recommended Dose | IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human MMP9 |
Antigen Species | Human |
Gene ID | 4318 |
Uniprot ID | |
Biology Area | Cardiovascular |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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