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Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930)

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Catalog No. TMAB-01659

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) is a Rabbit antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) can be used in WB.

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930)

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930)

😃Good
Catalog No. TMAB-01659
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) is a Rabbit antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) can be used in WB.
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50 μL$273 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: WB

Reactivity: SARS-CoV-2 WT,SARS-CoV-2 (D614G),SARS-CoV-2 Omicron and series of mutants
Ig Type
IgG
Clone
6F930
Reactivity
SARS-CoV-2 WT,SARS-CoV-2 (D614G),SARS-CoV-2 Omicron and series of mutants
Verified Activity
1. Sample:
Lane 1: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng
Lane 2: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng
Lane 3: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng
Lane 4: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng
Lane 5: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng
Lane 6: Recombinant SARS-CoV-2 N Protein (WT) at 10ng
Primary: Rabbit Anti-SARS-CoV-2 N protein (TMAB-01659) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:45.3 kDa
Observed band size:50 kDa
2. Sample:
Lane 1: SARS-CoV-2 N Protein (WT) at 10ng
Lane 2: SARS-CoV-2 N Protein (Del204 & Del215) at 2ng
Lane 3: SARS-CoV-2 N Protein (R203M & D377Y) at 2ng
Primary: Rabbit Anti-SARS-CoV-2 N Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:46 kDa
Observed band size:46 kDa
3. Sample:
Lane 1: SARS-CoV-2 N Protein (WT) at 10ng
Lane 2: SARS-CoV-2 N Protein (Del204 & Del215) at 10ng
Lane 3: SARS-CoV-2 N Protein (R203M & D377Y) at 10ng
Primary: Rabbit Anti-SARS-CoV-2 N Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:46 kDa
Observed band size:46 kDa
4. The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
5. The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationVirion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
Antigen Details
Immunogen
Recombinant Protein: SARS-CoV-2 Nucleocapsid Protein
Biology Area
SARS Coronavirus
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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