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Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930)
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Catalog No. TMAB-01659
Alias SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, Ormicron Nucleocapsid Protein, Ormicron N Protein, Ormicron mutant, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus Nucleocapsid Protein, novel coronavirus N Protein, 2019-nCoV Nucleoprotein, 2019-nCoV nucleocapsid protein, 2019-nCoV N Protein, 2019-nCoV N, 2019nCoV N, 2019 ncov N Protein
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) is a Rabbit antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) can be used in WB.
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930)
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930)
😃Good
Catalog No. TMAB-01659Alias SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, Ormicron Nucleocapsid Protein, Ormicron N Protein, Ormicron mutant, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus Nucleocapsid Protein, novel coronavirus N Protein, 2019-nCoV Nucleoprotein, 2019-nCoV nucleocapsid protein, 2019-nCoV N Protein, 2019-nCoV N, 2019nCoV N, 2019 ncov N Protein
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) is a Rabbit antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $273 | 7-10 days |
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| Description | Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) is a Rabbit antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) can be used in WB. |
| Synonyms | SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, Ormicron Nucleocapsid Protein, Ormicron N Protein, Ormicron mutant, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus Nucleocapsid Protein, novel coronavirus N Protein, 2019-nCoV Nucleoprotein, 2019-nCoV nucleocapsid protein, 2019-nCoV N Protein, 2019-nCoV N, 2019nCoV N, 2019 ncov N Protein |
| Ig Type | IgG |
| Clone | 6F930 |
| Reactivity | SARS-CoV-2 WT,SARS-CoV-2 (D614G),SARS-CoV-2 Omicron and series of mutants |
| Verified Activity | 1. Sample: Lane 1: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng Lane 2: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng Lane 3: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng Lane 4: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng Lane 5: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng Lane 6: Recombinant SARS-CoV-2 N Protein (WT) at 10ng Primary: Rabbit Anti-SARS-CoV-2 N protein (TMAB-01659) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:45.3 kDa Observed band size:50 kDa 2. Sample: Lane 1: SARS-CoV-2 N Protein (WT) at 10ng Lane 2: SARS-CoV-2 N Protein (Del204 & Del215) at 2ng Lane 3: SARS-CoV-2 N Protein (R203M & D377Y) at 2ng Primary: Rabbit Anti-SARS-CoV-2 N Protein Antibody at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:46 kDa Observed band size:46 kDa 3. Sample: Lane 1: SARS-CoV-2 N Protein (WT) at 10ng Lane 2: SARS-CoV-2 N Protein (Del204 & Del215) at 10ng Lane 3: SARS-CoV-2 N Protein (R203M & D377Y) at 10ng Primary: Rabbit Anti-SARS-CoV-2 N Protein Antibody at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:46 kDa Observed band size:46 kDa 4. The purity of the protein is greater than 90% as determined by reducing SDS-PAGE. 5. The purity of the protein is greater than 90% as determined by reducing SDS-PAGE. |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion. |
| Tissue Specificity | The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease t |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
| Immunogen | Recombinant Protein: SARS-CoV-2 Nucleocapsid Protein |
| Gene Name | S |
| Protein Name | Spike glycoprotein |
| Biology Area | SARS Coronavirus |
| Function | attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. |
| Molecular Weight | Theoretical: 46 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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