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Anti-PSGL-1/CD162 Antibody (2A36)

🥰Excellent
Catalog No. TMAY-02651

Anti-PSGL-1/CD162 Antibody (2A36) is a Rabbit antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (2A36) can be used in WB,ELISA,IP.

Anti-PSGL-1/CD162 Antibody (2A36)

Anti-PSGL-1/CD162 Antibody (2A36)

🥰Excellent
Catalog No. TMAY-02651
Anti-PSGL-1/CD162 Antibody (2A36) is a Rabbit antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (2A36) can be used in WB,ELISA,IP.
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Product Introduction

Bioactivity
Description
Anti-PSGL-1/CD162 Antibody (2A36) is a Rabbit antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (2A36) can be used in WB,ELISA,IP.
Ig Type
Monoclonal Rabbit IgG
Clone
2A36
Reactivity
Human
Verified Activity
1. SELPLG was immunoprecipitated using:

-Lane A:0.5 mg Jurkat Whole Cell Lysate.

-1 µL anti-SELPLG rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-SELPLG rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 43 kDa.

-Observed band size: 120 kDa.

2. Anti-SELPLG rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Jurkat Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:43 kDa.

-Observed band size:120 kDa(We are unsure as to the identity of these extra bands.)
Application
WB,ELISA,IP
Recommended Dose
WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human PSGL-1 / CD162 / SELPLG (rh PSGL-1 / CD162 / SELPLG; TMPY-03531; Q14242-1; Met1-Gly295).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundP-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human PSGL-1 / CD162 / SELPLG Protein (TMPY-03531)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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