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Anti-MBD1 Antibody (1J371)
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Catalog No. TMAH-00740
Alias Protein containing methyl-CpG-binding domain 1, PCM1, Methyl-CpG-binding protein MBD1, Methyl-CpG-binding domain protein 1, MBD 1, CXXC-type zinc finger protein 3, CXXC3
Anti-MBD1 Antibody (1J371) is an antibody targeting MBD1. Anti-MBD1 Antibody (1J371) can be used in ELISA,IF.
Anti-MBD1 Antibody (1J371)
Anti-MBD1 Antibody (1J371)
😃Good
Catalog No. TMAH-00740Alias Protein containing methyl-CpG-binding domain 1, PCM1, Methyl-CpG-binding protein MBD1, Methyl-CpG-binding domain protein 1, MBD 1, CXXC-type zinc finger protein 3, CXXC3
Anti-MBD1 Antibody (1J371) is an antibody targeting MBD1. Anti-MBD1 Antibody (1J371) can be used in ELISA,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $209 | 7-10 days | 7-10 days | |
| 100 μL | $347 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-MBD1 Antibody (1J371) is an antibody targeting MBD1. Anti-MBD1 Antibody (1J371) can be used in ELISA,IF. |
| Synonyms | Protein containing methyl-CpG-binding domain 1, PCM1, Methyl-CpG-binding protein MBD1, Methyl-CpG-binding domain protein 1, MBD 1, CXXC-type zinc finger protein 3, CXXC3 |
| Ig Type | Rabbit IgG |
| Clone | 1J371 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of MCF-7 cell with TMAH-00740 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 498-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Application | |
| Recommended Dose | IF:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Nucleus matrix. Nucleus speckle. Chromosome. |
| Tissue Specificity | Widely expressed. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human MBD1 |
| Antigen Species | Human |
| Gene Name | MBD1 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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