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Anti-PCYT1A Antibody (6F766) is an antibody targeting PCYT1A. Anti-PCYT1A Antibody (6F766) can be used in ELISA, WB, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
Alias | Phosphorylcholine transferase A, PCYT1, PCYT 1A, EC 2.7.7.15, CTPCT, CTP:phosphocholine cytidylyltransferase A, CT A, Choline-phosphate cytidylyltransferase A, CCT-alpha, CCT A |
Ig Type | Rabbit IgG |
Clone | 6F766 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: K562 whole cell lysate -All lanes: PCYT1A antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 42 kDa -Observed band size: 42 kDa 2. Immunofluorescence staining of Hela Cells with TMAH-00861 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, WB, IF |
Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Endoplasmic reticulum. Nucleus. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human PCYT1A |
Antigen Species | Human |
Gene ID | 5130 |
Uniprot ID | |
Biology Area | Neuroscience, Cancer, Metabolism, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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