CD40 Ligand Protein, Mouse, Recombinant (hFc)

Name: CD40 Ligand Protein, Mouse, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-02793
Rating: 4.5 (1 reviews)

(Synonyms: TRAP, Tnfsf5, T-BAM, Ly-62, Ly62, IMD3, IGM, HIGM1, gp39, CD40-L, Cd40l, CD40 ligand, CD154) Copy Product Info

Synonyms: TRAP, Tnfsf5, T-BAM, Ly-62, Ly62, IMD3, IGM, HIGM1, gp39, CD40-L, Cd40l, CD40 ligand, CD154

Catalog No. TMPY-02793 Copy Product Info

CD40 Ligand Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 44.4 kDa and the accession number is P27548.

Pack Size	Price	USA Stock	Global Stock
5 µg	$52	7-10 days	7-10 days
10 µg	$82	7-10 days	7-10 days
20 µg	$133	7-10 days	7-10 days
50 µg	$258	7-10 days	7-10 days
100 µg	$448	7-10 days	7-10 days
200 µg	$793	7-10 days	7-10 days
500 µg	$1,930	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Resource Download

User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	1.Measured by its binding ability in a functional ELISA. Immobilized Mouse CD40 His at 2 μg/ml (100 μl/well) can bind Mouse CD40 Ligand hFc, the EC50 of Mouse CD40 Ligand hFc is 60-300 ng/mL. 2.Loaded Recombinant Mouse CD40 Protein, His Tag on His1K Biosensor, can bind Recombinant Mouse CD40 Ligand Protein, hFc Tag with an affinity constant of 87.2nM as determined in BLI assay (Routinely tested).
Description	CD40 Ligand Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 44.4 kDa and the accession number is P27548.
Species	Mouse
Expression System	HEK293 Cells
Tag	N-hFc
Accession Number	P27548
Construction	A DNA sequence encoding the soluble form of mouse CD40LG (P27548) (Gly 115-Leu 260) was fused with the Fc region of human IgG1 at the N-terminus. Predicted N terminal: Glu
Protein Purity	> 80 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	TRAP, Tnfsf5, T-BAM, Ly-62, Ly62, IMD3, IGM, HIGM1, gp39, CD40-L, Cd40l, CD40 ligand, CD154
Research Background	The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

44.4 kDa (predicted); 50 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.