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MSLN/Mesothelin Protein, Human, Recombinant (His & Avi), Biotinylated

MSLN/Mesothelin Protein, Human, Recombinant (His & Avi), Biotinylated
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MSLN/Mesothelin Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00963
MSLN/Mesothelin Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 35.2 kDa and the accession number is Q13421-2.
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Pack SizePriceAvailabilityQuantity
100 μg$8147-10 days
500 μg$3,2507-10 days
1 mg$5,4407-10 days
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Biological Description

Biological Information
Immobilized Anti-MSLN Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MSLN, His Tag with the EC50 of 20.9ng/ml determined by ELISA.
Description
MSLN/Mesothelin Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 35.2 kDa and the accession number is Q13421-2.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberQ13421-2
Synonyms
SMR,MSLN,CAK1,MPF,Mesothelin,MPFSMRP
Construction
Glu296-Gly580
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight35.2 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.

Keywords