BTN3A1 Protein, Human, Recombinant

Name: BTN3A1 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-06663
Rating: 4.5 (1 reviews)

(Synonyms: CD277, butyrophilin subfamily 3 member A1, BTN3.1, BTF5, BT3.1) Copy Product Info

Synonyms: CD277, butyrophilin subfamily 3 member A1, BTN3.1, BTF5, BT3.1

Catalog No. TMPY-06663 Copy Product Info

BTN3A1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.16 kDa and the accession number is NP_919423.1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$85	7-10 days	7-10 days
10 µg	$146	7-10 days	7-10 days
20 µg	$242	7-10 days	7-10 days
50 µg	$477	7-10 days	7-10 days
100 µg	$929	7-10 days	7-10 days
200 µg	$1,390	7-10 days	7-10 days
500 µg	$2,930	7-10 days	7-10 days
1 mg	$5,930	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human BTN3A1, No Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 8.8 ng/ml determined by ELISA.
Description	BTN3A1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.16 kDa and the accession number is NP_919423.1.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	O00481-1
Construction	Gln30-Gly254
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	CD277, butyrophilin subfamily 3 member A1, BTN3.1, BTF5, BT3.1
Research Background	BTN3A1 has the structure of a type I receptor of the Ig superfamily and is part of a family of seven BTN receptors encoded by genes in the MHC. BTN molecules are composed of two Ig domains (IgV, IgC2), a single transmembrane domain, and a large carboxyl-terminal domain termed B3.2 (or PRYSPRY) located in the cell cytoplasm. There are three human BTN3A loci, BTN3A1, BTN3A2, and BTN3A3, and clear orthologs of BTN3A molecules, now called CD277, are absent from the mouse genome. Despite its similarity to B7 molecules, BTN3A1 was proposed to act not as a coreceptor or costimulatory molecule, but rather to directly present pAg to the γδ TCR in a manner analogous to MHC-restricted peptide presentation. However, this model of BTN3A1 function has been challenged by conflicting data, which show pAg binding to a positively charged pocket in the cytosolic B3.2 domain, and that BTN3A1 does not directly engage the γδ TCR. This contradictory picture has emerged as a result of the complexity of the system and in particular by the use of endogenous and exogenous routes of Ag delivery in in vitro assays.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

24.15 kDa (predicted); 26-32 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.