Ephrin B2/EFNB2 Protein, Human, Recombinant (hFc)

Catalog No. TMPK-00223 Copy Product Info

Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.

Ephrin B2/EFNB2 Protein, Human, Recombinant (hFc)

Catalog No. TMPK-00223

Copy Product Info

Ephrin B2/EFNB2 Protein, Human, Recombinant (hFc)

Pack Size	Price	USA Stock	Global Stock
5 μg	$30	-	In Stock
10 μg	$44	-	In Stock
20 μg	$67	-	In Stock
50 μg	$126	-	In Stock
100 μg	$207	In Stock	In Stock
200 μg	$376	7-10 days	7-10 days
500 μg	$838	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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COA User Guide of Recombinant Proteins

Product Information

Biological Activity	Immobilized Human EPHB2, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2 ng/mL determined by ELISA (QC Test). Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
Description	Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P52799
Synonyms	LERK5,HTKL,EPLG5,Ephrin B2,EFNB2
Construction	Ile28-Ala229
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	49 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.