EphA2 Protein, Mouse, Recombinant

Name: EphA2 Protein, Mouse, Recombinant
Brand: TargetMol
SKU: TMPY-06635
Rating: 4.5 (1 reviews)

(Synonyms: Sek-2, Sek2, Myk2, EPH receptor A2, Eck, AW545284) Copy Product Info

Synonyms: Sek-2, Sek2, Myk2, EPH receptor A2, Eck, AW545284

Catalog No. TMPY-06635 Copy Product Info

EphA2 Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.03 kDa and the accession number is NP_034269.2.

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days
200 µg	$1,230	7-10 days	7-10 days
500 µg	$2,580	7-10 days	7-10 days
1 mg	$5,580	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Recombinant Mouse EphA2 Protein (ECD) at 2 μg/ml (100 μl/well) can bind Recombinant Human Ephrin-A1 / EFNA1 Protein (Fc Tag), The EC50 is 1.8-5.4 ng/mL.
Description	EphA2 Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.03 kDa and the accession number is NP_034269.2.
Species	Mouse
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	NP_034269.2
Construction	A DNA sequence encoding the mouse EphA2 (NP_034269.2) (Met1-Asn535) was expressed. Predicted N terminal: Ala 20
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	Sek-2, Sek2, Myk2, EPH receptor A2, Eck, AW545284
Research Background	Eph receptor A2 (Ephrin type-A receptor 2 or EphA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptors' corresponding family of ligands are the ephrins anchored to cell surfaces. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. They have also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. The large family of ligands and receptors may make a major contribution to the accurate spatial patterning of connections and cell position in the nervous system. Furthermore, elevated expression of Eph receptors and ephrin ligands is associated with tumors and associated tumor vasculature, suggesting the Eph receptors and ephrin ligands also play critical roles in tumor angiogenesis and tumor growth. Unlike most Eph kinases, which are primarily expressed during development, EphA2 is primarily found in adult human epithelial cells. The cellular functions of EphA2 may be regulating cell growth, survival, migration, and angiogenesis.Unlike other receptor tyrosine kinases, ligand binding is not necessary for EphA2. Rather, the ligand appears to regulate EphA2 subcellular localization and its interactions with downstream adapter and signaling proteins. Eph receptor A2(EphA2) has been demonstrated to critically regulate tumor cell growth, migration and invasiveness. Eph receptor A2(EphA2) is frequently overexpressed and functionally altered in aggressive tumor cells, and that these changes promote metastatic character.

Chemical Properties

Molecular Weight

57.03 kDa (predicted); 61.90 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.