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Anti-ILF3 Antibody (8H129)
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Catalog No. TMAH-00630
Alias Translational control protein 80, TCP80, Nuclear factor of activated T-cells 90 kDa, Nuclear factor associated with dsRNA, NF-AT-90, NFAR, MPP4, M-phase phosphoprotein 4, Interleukin enhancer-binding factor 3, ILF 3, DRBP76, DRBF MPHOSPH4 NF90, Double-stranded RNA-binding protein 76
Anti-ILF3 Antibody (8H129) is an antibody targeting ILF3. Anti-ILF3 Antibody (8H129) can be used in ELISA, IHC, IF.
Anti-ILF3 Antibody (8H129)
Anti-ILF3 Antibody (8H129)
😃Good
Catalog No. TMAH-00630Alias Translational control protein 80, TCP80, Nuclear factor of activated T-cells 90 kDa, Nuclear factor associated with dsRNA, NF-AT-90, NFAR, MPP4, M-phase phosphoprotein 4, Interleukin enhancer-binding factor 3, ILF 3, DRBP76, DRBF MPHOSPH4 NF90, Double-stranded RNA-binding protein 76
Anti-ILF3 Antibody (8H129) is an antibody targeting ILF3. Anti-ILF3 Antibody (8H129) can be used in ELISA, IHC, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | 197 € | 7-10 days | |
| 100 μL | 330 € | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-ILF3 Antibody (8H129) is an antibody targeting ILF3. Anti-ILF3 Antibody (8H129) can be used in ELISA, IHC, IF. |
| Alias | Translational control protein 80, TCP80, Nuclear factor of activated T-cells 90 kDa, Nuclear factor associated with dsRNA, NF-AT-90, NFAR, MPP4, M-phase phosphoprotein 4, Interleukin enhancer-binding factor 3, ILF 3, DRBP76, DRBF MPHOSPH4 NF90, Double-stranded RNA-binding protein 76 |
| Ig Type | Rabbit IgG |
| Clone | 8H129 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00630 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. IHC image of TMAH-00630 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Immunofluorescence staining of Hela Cells with TMAH-00630 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, IHC, IF |
| Recommended Dose | IHC:1:50-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus, nucleolus. Cytoplasm. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs. As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response. Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human ILF3 |
| Antigen Species | Human |
| Gene ID | 3609 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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