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Anti-MERTK/Mer Antibody (5M877)
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Catalog No. TMAH-00745
Alias Tyro12, RP38, MGC133349, MERTK, MER, c-mer, c-Eyk
Anti-MERTK/Mer Antibody (5M877) is a Mouse antibody targeting MERTK/Mer. Anti-MERTK/Mer Antibody (5M877) can be used in ELISA, WB, IF, FCM.
Anti-MERTK/Mer Antibody (5M877)
Anti-MERTK/Mer Antibody (5M877)
😃Good
Catalog No. TMAH-00745Alias Tyro12, RP38, MGC133349, MERTK, MER, c-mer, c-Eyk
Anti-MERTK/Mer Antibody (5M877) is a Mouse antibody targeting MERTK/Mer. Anti-MERTK/Mer Antibody (5M877) can be used in ELISA, WB, IF, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, WB, IF, FCM Reactivity: Human |
| Alias | Tyro12, RP38, MGC133349, MERTK, MER, c-mer, c-Eyk |
| Ig Type | IgG2b |
| Clone | 5M877 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: 1-4 lanes: 293T whole cell lysate transfected with MERTK at 10ug, 5ug, 2.5ug, 1.25ug -All lanes: MERTK antibody at 1:2000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 150-250 KDa -Observed band size: 150-250 KDa -Exposure time：5S 2. Western Blot -Positive WB detected in: 1-4 lanes: Recombinant MERTK protein at 20ng, 10ng, 5ng, 2.5ng -All lanes: MERTK antibody at 1:2000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 120 KDa -Observed band size: 120-150 KDa -Exposure time：5S 3. Western Blot -Positive WB detected in: 293 whole cell lysate, SY5Y whole cell lysate, K562 whole cell lysate -All lanes: MERTK antibody at 1:1000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 110 kDa -Observed band size: 130 KDa -Exposure time：5min 4. Immunofluorescence staining of 293T transfected cells with MERTK TMAH-00745 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). 5. Immunofluorescence staining of HepG2 cells with MERTK TMAH-00745 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). 6. Overlay Peak curve showing 293T transfected cells stained with TMAH-00745 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, IF, FCM |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purity | >95% |
| Research Background | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: MERTK Protein (21-505AA) |
| Antigen Species | Human |
| Gene ID | 10461 |
| Uniprot ID | |
| Biology Area | Signal Transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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