IL-12B Protein, Human, Recombinant (hFc)

IL-12B Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 61.4 kDa and the accession number is P29460.

Pack Size	Availability	Price/USD	Quantity
50 μg	5 days	$ 383.00
500 μg	5 days	$ 2,510.00

Pack Size

Availability

Price/USD

Quantity

50 μg

5 days

$ 383.00

500 μg

5 days

$ 2,510.00

Description

IL-12B Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 61.4 kDa and the accession number is P29460.

Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P29460
Synonyms	IMD29, IL-12B, interleukin 12B, CLMF2, IL12 p40, NKSF2, CLMF, IMD28, NKSF
Construction	The p40 subunit of Human IL12, termed asIL12B (NP_002178.2) (Met 1-Ser 328) was fused with the Fc region ofHuman IgG1 at the C-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
Molecular Weight	61.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor-like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine is important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine that functions in innate and adaptive immunity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy